Immunoglobulin gene rearrangements are formed from the earliest stages of B-cell and T-cell development onwards. Random coupling between one of many V, (D) and J genes results in the formation of a unique V(D)J exon that encodes the actual antigen-binding moiety of the Ig or TCR chain. Owing to the huge diversity in Ig/TCR rearrangements, the diversity of different Ig or TCR molecules is estimated to be in the order of 1012. Consequently each lymphocyte has a unique antigen receptor molecule on its membrane and the chance that two different lymphocytes coincidentally bear the same receptor is almost negligible. Hence, identical rearrangements are not derived from multiple independently generated cells, but rather reflect the clonal nature of the involved cell population.
B-cell clonality analysis targets immunoglobulin (Ig) genes (Ig heavy chain, IGH; Ig kappa light chain, IGK. Clonality analysis of lymphocyte populations relies on fragment size analysis of PCR amplified rearranged antigen receptor genes.
Clinical Indications For diagnostic testing in lymphoid malignancies such as lymphomas.
Sample Required A minimum of 4ml Blood or 1 ml bone marrow in EDTA (lavender/purple top) within 72 hours of collection at room temperature
Sample Collection Please ensure the sample is sent to the lab ASAP after collection as it must be received within 72 hours.