Test Background An antibody screen, including smooth muscle, gastric parietal cell, mitochondrial and LKM antibodies, by indirect immunofluorescence using mouse liver/kidney/stomach substrate. Positive samples are then confirmed by a secondary method. Smooth muscle antibodies at a high titre are associated with chronic active hepatitis, and at low titres are more likely to be triggered by infection. Mitochondrial antibodies are associated with primary biliary cirrhosis. Gastric parietal cell antibodies suggest pernicious anaemia (PA), atrophic gastritis; they are present in the early stages of PA, and frequently diminish with disease progression.
Clinical Indications Autoimmune liver disease Low vitamin B12, suspected pernicious anaemia Autoimmune gastritis Autoimmune hepatitis Primary biliary cirrhosis
Reference Range Negative
Sample Required RST (rust top)
Sample Volume 5-10 mL (1 mL serum)
Turnaround Time 7 days
Notes This test is currently unaccredited due to a recent change in platform.