Imperial Collage Healthcare

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Direct antiglobulin test

Test Background
To detect the presence of IgG antibodies bound to the antigens on the red blood cell surface membrane.

Clinical Indications
To determine if there are any antibodies on the surface of the patient's red cells. Used in the investigation of haemolytic anaemia, a haemolytic transfusion reaction and drug-induced haemolysis.

Reference Range

Not applicable

Sample Required
EDTA (lavender top)

Sample Volume
4 mL for paediatrics6 mL for adults

Turnaround Time
Dependent on clinical requirement. Contact the laboratory to discuss further.


May be referred to the NHS Blood and Transplant service for additional testing

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