Imperial Collage Healthcare

Group and Antibody Screen

Test Background

Used to identify a person’s ABO and Rh D blood group and antibody status. The test is essential for patients who may require a blood transfusion.

Clinical Indications
To Establish the ABO and D group prior to transfusion or in pregnancy

To detect circulating antibodies to red cell antigens in the patients plasma

Reference Range

Not applicable

Sample Required
EDTA (pink top)

Sample Volume
1 mL for < 4 months old
4 mL for paediatrics
6 mL for adults


There must be different tubes for blood grouping and FBC- samples will not be shared.

A maternal sample may also be required if transfusion is planned (neonatal requests)

May be referred to the NHS Blood and Transplant service for confirmation if necessary.

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