Platelet function is initially assessed using the PFA-100 analyser which offers quantitative assessment of platelet function at high shear as whole blood is forced through an aperture coated with either collagen/epinephrine or collagen/ADP agonists. The test is measured as aperture closure time (CT) in seconds for each COL/EPI and COL/ADP cartridge.
The PFA is sensitive to platelet function defects such as Glanzmann’s Thrombasthaenia and Bernard-Soulier Syndrome as well as disorders of von Willebrand factor, though sensitivity is reduced when VWD Type I is included. Aspirin or aspirin-like platelet defects are associated with a significantly prolonged COL/EPI closure time.
Investigation of bleeding phenotype suggestive of possible platelet function disorder
Confirmation of previously diagnosed platelet function disorder
Collagen/Epinephrine: 75 – 165 seconds
Collagen/ADP: 70 – 120 seconds
Sample Required 4.5 mL sodium citrate (pale blue top) adults 1.8 mL sodium citrate (pale blue top) paediatrics
Turnaround Time 4 hours
Please note: Samples will be rejected if under/over-filled, clotted or haemolysed
Please do not send samples via pod system