Imperial Collage Healthcare

Anti-TNF drug levels Anti- TNF drug neutralising antibodies (Infliximab IFX, Adalimumab ADA, Etanercept ETA)


Test Background

A significant proportion of patients treated with anti-TNF therapy are primary non-responders and require dose- or dose frequency adjustments. The lack of response to treatment has also been attributed to immunogenicity and the development of anti-drug neutralising antibodies. Studies have shown that response rates increase with infliximab trough concentration and serum drug levels at 4 weeks post-infusion in some patients are positively correlated with duration of response.  However the probability of an infection increases with the number of anti-TNF infusions. Anti-TNF drugs can therefore be considered to have a narrow therapeutic index and drug monitoring based on serum trough levels together with monitoring for anti-drug antibodies (neutralising antibodies) may improve clinical treatment decisions.

An in-house ELISA assay is used to measure serum trough drug levels. Neutralising antibodies are measured using the ILite NAb bioassay (BioMonitor) which detects antibodies in serum with the ability to neutralise anti-TNF drugs.


Clinical Indications
For the monitoring of patients treated with Infliximab, Adalimumab, or Etanercept. (Please note we will only measure neutralising antibodies when drug trough levels are below 0.5ug/ml).


Reference Range

NA


Sample Required
Serum - RST (rust top gel)


Sample Collection
Perform venesection BEFORE next drug infusion


Sample Volume
5ml


Transport/Storage

Room temperature


Turnaround Time

Drug levels = 2 weeks

Neutralising antibodies = 4 weeks


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