Test BackgroundThis is a two-part test, performed by complement dependent cytotoxicity (CDC) and flow cytometry.
- HLA crossmatching tests recipients’ serum for the presence of anti-donor antibodies which may represent a risk or contraindication to transplantation, particularly in solid organ grafts. Preformed IgG, or occasionally IgM antibodies, in the recipient, directed against donor HLA antigens, are a cause of hyperacute or accelerated allograft rejection.
- CDC detects IgG and IgM complement fixing antibodies that are likely to cause antibody-mediated rejection. FCXM does not rely on complement-dependent cytotoxicity and can therefore detect non-complement fixing IgG1 and IgG3 antibodies as well. It is significantly more sensitive than the CDC crossmatch and is of particular benefit prior to transplantation of sensitised recipients or recipients of second or subsequent allografts. It is important in these tests to provide very clear clinical details, including the relationship of the potential donor to the recipient.
|Interpretive report provided
Sample Required EDTA (lavender top) donorSerum (red top) preferred, SST (gold top) acceptable recipient
Sample Volume 20 mL donor10 mL recipient
Turnaround Time Please consult the laboratory
NotesBOTH samples must be <24 hours old