Imperial Collage Healthcare

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HLA antibody testing

Lab Code : CABS/HLA Abs/DSAbs

Test Background
The laboratory employs a number of alternative assay systems with varying sensitivity and clinical significance. In most cases, routine screening will be performed using one, or a combination, of complement dependent cytotoxicity, ELISA, flow cytometry or Luminex technology. Post transplant monitoring is usually performed using the Luminex Single Antigen (SA) analysis, a powerful technique used to detect the presence of donor-specific antibodies (DSA).

Clinical Indications
Used for the detection and characterisation of lymphocytotoxic antibodies in patients awaiting renal or pancreas transplant, or those who have received a transplant. Also used in the investigation of transfusion reactions. For renal/pancreas recipients, patient antibody profiles are maintained by the laboratory.

Sample Required
Serum (red top) preferred, SST (gold top) acceptable

Sample Volume
10 mL

Turnaround Time
Please consult the laboratory

Samples must be sent via first class post or courier. It is important to record the sample date when requesting these tests. Please provide HIV and hepatitis B status. Results are held with the patient history, and are not routinely reported for individual samples.

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