Lab Code : ENAS
Test Background This test detects antibodies against a group of antigens in the nucleus and cytoplasm. ENA antibodies are useful in classifying clinical subsets of connective tissue diseases. The screen includes: anti-Ro (SS-A) and anti-La (SS-B) antibodies seen in Sjögren’s and SLE; anti-Sm antibody specific for SLE; anti-ribonucleoprotein (RNP) antibodies seen in mixed connective tissue disease; anti-Jo-1 (histidyl-tRNA synthetase) antibody seen in myositis and anti-Scl-70 (topoisomerase-1) antibody seen in scleroderma.
Clinical Indications Suspected autoimmune-mediated connective tissue disorders ‘Speckled’ pattern observed on antinuclear antibody immunofluorescence
Reference Range Negative Positive range: 0-40 AU/mL
Sample Required RST (rust top)
Sample Volume 5-10 mL (1 mL serum)
Turnaround Time 10 days screen 14 days specificities
Notes Due to a change in equipment, this test is awaiting accreditation