Imperial Collage Healthcare

Complement, total classical pathway (THC)


Test Background
The CH50 assay measures the ability of serum complement components to form membrane attack complex, which can be detected by ELISA.
C3 and C4 levels must also be requested.
The assay is used as a screening test for defects of individual components of the classical and terminal pathways. It should not be used for monitoring complement consumption, instead request C3 and C4 levels.


Clinical Indications

  • Suspected complement deficiency
  • Recurrent infections


Reference Range

50-125 % of normal


Sample Required
RST (rust top)
Separate and freeze immediately
Transport frozen


Sample Volume
5-10 mL (1 mL serum)


Turnaround Time
21 days


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