Imperial Collage Healthcare

Antinuclear antibody screen

Test Background
Commonly positive at high titre (>1:640) in patients with SLE and other connective tissue diseases. The presence of ANA is indicative of SLE (present in 80-90 % of cases) although they also appear in some other autoimmune diseases, such as Sjögren’s syndrome (60 %), rheumatoid arthritis, autoimmune hepatitis, scleroderma and poly/dermatomyositis, Hashimoto’s disease and autoimmune haemolytic anaemia. Weak positive reactions are not uncommon and may have no clinical significance.

Clinical Indications

  • ANA should be requested for investigations of suspected connective tissue autoimmune syndromes. Autoantibody screening should be reserved for investigations where there is suspected liver involvement and/or anaemia. Autoimmune symptoms are typically variable and include:
  • painful or swollen joints
  • unexplained fever
  • back pain
  • extreme fatigue
  • rashes

Reference Range


Sample Required
RST (rust top)

Sample Volume
5-10 mL (1 mL serum)

Turnaround Time
4 days

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