Test BackgroundImmunophenotyping tests are used to diagnose and classify suspected haematological abnormalities involving white blood cells. This test is vital in supplementing tests undertaken in cytogenetics and histopathology to provide further information on cell type and maturation. This information guides clinicians in formulating specific and effective treatment plans.
Clinical Indications The immunophenotyping laboratory performs flow cytometric analysis for a wide variety of disorders, including: acute myeloid leukaemia (AML) acute lymphoblastic leukaemia (ALL) myeloproliferative disorders (MPD) chronic lymphocytic leukaemia (CLL) myelodysplastic syndromes (MDS) aplastic anaemia and other pancytopenias plasma cell disorders lymphocytosis investigation of CSF samples or effusions investigation of paroxysmal nocturnal haemoglobinuria.
Sample Required Blood or bone marrow in EDTA (lavender top) are usually the samples of choice, although CSF, pleural fluid, ascetic fluid can also be sent in universal containers.
Investigation of PNH is performed only on blood in EDTA (1 mL minimum).
Sample Volume Samples would not be rejected on the basis of small volume, however, approximately 1 mL is ideal.
Urgent referrals – 95% within 1 calendar day
Semi-Urgent - 95% within 3 calendar days
Routine - 95% within 7 calendar days