The chimerism investigation is carried out following haematopoietic stem cell transplants to monitor the proportion of host and donor DNA. A semi-quantitative amplification of informative microsatellites in whole blood and T-cell separated samples is undertaken to obtain a value to indicate the donor recipients transplant status.
Purified DNA is amplified via PCR using a set of 15 fluorescent-labelled primer pairs (in one reaction tube multiplex-PCR), specific for amplification of 15 microsatellite markers. Where the amplified products are different sizes (alleles) between donor and host for the same marker, it is possible to discriminate between donor and host DNA. This discrimination of the amount of donor DNA present in the host, thereby providing semi-quantitative data for monitoring level of engraftment.
Clinical Indications Monitoring engraftment in Post-BMT patients - undertaking an analysis of chimerism is a valuable tool in assessing a patient’s response to transplantation. By observing the chimeric profiles and assessing the donor and recipient ratios, it is possible to determine the success of the transplant and if there is progression or potential to relapse.
Sample Required >10 mL peripheral blood or 3-5.0 mL bone marrow in EDTA (lavender top)
Sample Container EDTA (lavender/purple top)
Sample Volume 1ml blood or bone marrow
Transport/Storage Samples must be less than 72 hours old upon receipt in the lab.