Imperial Collage Healthcare

Dye binding


Test Background
The fluorescent dye EMA (eosin-5-maleimide ) is used to label band 3 protein, which indirectly interacts with the spectrin-based cytoskeleton and membrane lipid bilayer in red blood cells.
Deficiency or abnormality of band 3 is seen in hereditary spherocytosis red cells, and has also been observed in cases of Southeast Asian ovalocytosis, congenital dyserythropoietic anaemia type II, and the cryohydrocytosis variants of stomatocytosis.


Clinical Indications

  • Unexplained haemolysis
  • Spherocytosis
  • Suspected hereditary spherocytosis


Reference Range

0.83-1.17 (ratio)


Sample Required
4 mL EDTA (lavender top) adults
0.5 mL EDTA (lavender top) paediatrics


Sample Volume
0.5 mL adult tube
0.2 mL paediatric tube


Turnaround Time
Within 4 working days


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