Donath-Lansteiner test (for paroxysmal cold haemoglobinuria)
Test Background Paroxysmal cold haemoglobinuria (PCH) is an autoimmune haemolytic anaemia caused by cold-reacting immunoglobulins. It primarily affects children and tends to cause quite severe, but transient, disease.
Clinical Indications Presence of a biphasic antibody that binds to the red cells in the cold and lyses the red cell at 37 °C.
Sample Required EDTA (lavender top)
Sample Volume 4 mL for paediatrics6 mL for adults
Turnaround Time Dependent on clinical requirement. Contact the laboratory to discuss further.
Notes This test is referred to the NHS Blood and Transplant service